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e2f 4  (Biorbyt)


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    Biorbyt e2f 4
    E2f 4, supplied by Biorbyt, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f+4/E2F4+antibody/pm39617889-357-153-154
    Average 92 stars, based on 1 article reviews
    e2f 4 - by Bioz Stars, 2026-09
    92/100 stars

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    Transduction:

    Article Title: Functional screening reveals genetic dependencies and diverging cell cycle control in atypical teratoid rhabdoid tumors
    Article Snippet: .. Primary antibodies used in this study for DigiWest were the following: β-actin (Sigma, A1978, AMBRA1 (Cell Signaling, 24907), ATM (Cell Signaling, 2873), ATR (Cell Signaling, 2790), AURKA (Cell Signaling, 4718), AURKB (Cell Signaling, 3094), BUB1B (Cell Signaling, 5421), Caspase3 (Cell Signaling, 9662), CDK1 (Cell Signaling, 9112), CDK1-pTyr15 (Cell Signaling, 4539), CDC25A (abm, Y021163), CDC25A-pSer75 (abm, Y011138), CDC25B (R&D, AF1649), CDC27 (Transduction Laboratories, C40920), CDK2 (Cell Signaling, 2546), CDK2-pThr160 (Cell Signaling, 2561), CDK3 (abcam, ab135805), CDK4 (Cell Signaling, 2906), CDK4-pThr172 (Invitrogen, PA-64482), CDK5 (Cell Signaling, 2506), CDK6 (Cell Signaling, 13331), CDK6-pTyr13 (biorbyt, orb15013), CDK6-pTyr24 (biorbyt, orb15014), CHK2 (Cell Signaling, 3440), CHK2-pThr68 (Cell Signaling, 2661), c-MYC (Cell Signaling, 9402), c-MYC-pThr58 (ThermoFisher, PA5-37654), c-MYC-pThr62/Ser62 (abcam, ab32029), cyclin A (abcam, ab53054), cyclin B1 (abcam, ab32053), cyclin D1 (Cell Signaling, 2926), cyclin D1-pThr286 (ThermoFisher, PA5-37487), cyclin D2 (Cell Signaling, 3741), cyclin D3 (Cell Signaling, 2936), cyclin E1 (Cell Signaling, 4129), cyclin E2 (Cell Signaling, 4132), E2F-2 (Millipore, DR1095), E2F-4 (biorbyt, orb10571), histone H3-pSer28 (Millipore, 07–145), hisotne H3-pSer10 (Cell Signaling, 9701), MCM2 (Cell Signaling, 3619), MCM2-pSer139 (Cell Signaling, 8861), MDM2 (Santa Cruz, sc-965), MDM2-pSer166 (Cell Signaling, 3521), p16 (ProteinTech Group, 10883-1-AP), p21 (Cell Signaling, 2947), p27 (Cell Signaling, 3698), p53 (Santa Cruz, sc-126), p53-pSer37 (Cell Signaling, 9289), p53-pSer15 (Cell Signaling, 9284), RB (Cell Signaling, 9313), RB-pSer807/Ser811 (Cell Signaling, 8516), RB-pSer780 (Cell Signaling, 3590), RB-pSer608 (Cell Signaling, 8147), RB-pSer795 (Cell Signaling, 9301), RBPSUH (Cell Signaling, 5313), RPA2 p34 (Millipore, 04–1481), Survivin (Cell Signaling, 2802), TOPK (Cell Signaling, 4942), and TOPO 2 alpha (Santa Cruz, sc-13058). .. In order to overexpress either 3xFLAG tag alone or 3xFLAG-AMBRA1 in ATRT cells, we first generated lentiviral plasmids N174-MCS (Puro) (Addgene #81068) carrying either 3xFLAG or 3xFLAG-AMBRA1.

    Article Title: Functional screening reveals genetic dependencies and diverging cell cycle control in atypical teratoid rhabdoid tumors.
    Article Snippet: .. Primary antibodies used in this study for DigiWest were the following: β-actin (Sigma, A1978, AMBRA1 (Cell Signaling, 24907), ATM (Cell Signaling, 2873), ATR (Cell Signaling, 2790), AURKA (Cell Signaling, 4718), AURKB (Cell Signaling, 3094), BUB1B (Cell Signaling, 5421), Caspase3 (Cell Signaling, 9662), CDK1 (Cell Signaling, 9112), CDK1-pTyr15 (Cell Signaling, 4539), CDC25A (abm, Y021163), CDC25A-pSer75 (abm, Y011138), CDC25B (R&D, AF1649), CDC27 (Transduction Laboratories, C40920), CDK2 (Cell Signaling, 2546), CDK2-pThr160 (Cell Signaling, 2561), CDK3 (abcam, ab135805), CDK4 (Cell Signaling, 2906), CDK4-pThr172 (Invitrogen, PA-64482), CDK5 (Cell Signaling, 2506), CDK6 (Cell Signaling, 13331), CDK6-pTyr13 (biorbyt, orb15013), CDK6-pTyr24 (biorbyt, orb15014), CHK2 (Cell Signaling, 3440), CHK2-pThr68 (Cell Signaling, 2661), c-MYC (Cell Signaling, 9402), c-MYC-pThr58 (ThermoFisher, PA5-37654), c-MYC-pThr62/Ser62 (abcam, ab32029), cyclin A (abcam, ab53054), cyclin B1 (abcam, ab32053), cyclin D1 (Cell Signaling, 2926), cyclin D1-pThr286 (ThermoFisher, PA5-37487), cyclin D2 (Cell Signaling, 3741), cyclin D3 (Cell Signaling, 2936), cyclin E1 (Cell Signaling, 4129), cyclin E2 (Cell Signaling, 4132), E2F-2 (Millipore, DR1095), E2F-4 (biorbyt, orb10571), histone H3-pSer28 (Millipore, 07–145), hisotne H3-pSer10 (Cell Signaling, 9701), MCM2 (Cell Signaling, 3619), MCM2-pSer139 (Cell Signaling, 8861), MDM2 (Santa Cruz, sc-965), MDM2-pSer166 (Cell Signaling, 3521), p16 (ProteinTech Group, 10883-1-AP), p21 (Cell Signaling, 2947), p27 (Cell Signaling, 3698), p53 (Santa Cruz, sc-126), p53-pSer37 (Cell Signaling, 9289), p53-pSer15 (Cell Signaling, 9284), RB (Cell Signaling, 9313), RB-pSer807/Ser811 (Cell Signaling, 8516), RB-pSer780 (Cell Signaling, 3590), RB-pSer608 (Cell Signaling, 8147), RB-pSer795 (Cell Signaling, 9301), RBPSUH (Cell Signaling, 5313), RPA2 p34 (Millipore, 04–1481), Survivin (Cell Signaling, 2802), TOPK (Cell Signaling, 4942), and TOPO 2 alpha (Santa Cruz, sc-13058). .. In order to overexpress either 3xFLAG tag alone or 3xFLAG-AMBRA1 in ATRT cells, we first generated lentiviral plasmids N174-MCS (Puro) (Addgene #81068) carrying either 3xFLAG or 3xFLAG-AMBRA1.



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    (A) GESA plot with normalized enrichment score (NES) of REACTOME gene sets. NES was analyzed between MCF7 and p53KO treated with 50 nM abemaciclib at day21. REACTOME-E2F-mediated-regulation-of-DNA-replication (top), REACTOME-Transcription-of-E2F-targets-under-negative-control-by-DREAM-complex (middle) and REACTOME-Transcription-of-E2F-targets-under-negative-control-byp107 RBL1-and-p130 RBL2-in-complex-with-HDAC1 (bottom). (B) Immunoblotting of DREAM complex components with 50 nM abemaciclib. (C) Cells were treated with 100 nM abemaciclib for 48 h. Lysates were immunoprecipitated with p130 and IgG antibodies. (D) Cells were treated with 7 days of abamacilib and tested by ChIP followed by real-time PCR. Bar showed technical duplicate. Unpaired, Student’s t test. (E) Western blot results of MCF7 cells and p53KO transduced with doxycycline (dox)-inducible HA-tagged p21. The cells were treated with 50 nM abemaciclib or DMSO +/− dox for 48 h. (F) Dox-inducible HA-p21 MCF7 and p53KO cells were treated with 100 nM abemaciclib and +/− dox for 72 h. Lysates were immunoprecipitated with <t>E2F4</t> and IgG antibodies. (G) Time course change of the proportion of G1 phase after drug withdrawal. FUCCI labeled MCF7 were treated with 50 nM abemaciclib and FUCCI labeled p53KO cells were treated with or without dox and with 50 nM abemaciclib for 4 days. The cells were cultured without abemaciclib for 62 h under time-lapse imaging. Two-way ANOVA, Tukey’s. (H) Cell viability treated with 50 nM abemaciclib or DMSO +/− dox. Data are means ± SEM of four replicates. Two-way ANOVA, Tukey’s. (I) Flow cytometry analysis showing the percentage of SA-β-Gal positive cells. Data are means ± SD of three replicates. Two-way ANOVA, Sidak’s. (J) Colony formation assay. Cells were treated with 50 nM abemaciclib ± dox for 11 days and reseeded without drug. (K) Cell viability of p130KO in dox inducible HA-p21 cells. The cells were treated with 50 nM abemaciclib or DMSO +/− dox. Data are means ± SEM of six replicates. Two-way ANOVA, Tukey’s. See also and .
    E2f4 Ip, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) GESA plot with normalized enrichment score (NES) of REACTOME gene sets. NES was analyzed between MCF7 and p53KO treated with 50 nM abemaciclib at day21. REACTOME-E2F-mediated-regulation-of-DNA-replication (top), REACTOME-Transcription-of-E2F-targets-under-negative-control-by-DREAM-complex (middle) and REACTOME-Transcription-of-E2F-targets-under-negative-control-byp107 RBL1-and-p130 RBL2-in-complex-with-HDAC1 (bottom). (B) Immunoblotting of DREAM complex components with 50 nM abemaciclib. (C) Cells were treated with 100 nM abemaciclib for 48 h. Lysates were immunoprecipitated with p130 and IgG antibodies. (D) Cells were treated with 7 days of abamacilib and tested by ChIP followed by real-time PCR. Bar showed technical duplicate. Unpaired, Student’s t test. (E) Western blot results of MCF7 cells and p53KO transduced with doxycycline (dox)-inducible HA-tagged p21. The cells were treated with 50 nM abemaciclib or DMSO +/− dox for 48 h. (F) Dox-inducible HA-p21 MCF7 and p53KO cells were treated with 100 nM abemaciclib and +/− dox for 72 h. Lysates were immunoprecipitated with E2F4 and IgG antibodies. (G) Time course change of the proportion of G1 phase after drug withdrawal. FUCCI labeled MCF7 were treated with 50 nM abemaciclib and FUCCI labeled p53KO cells were treated with or without dox and with 50 nM abemaciclib for 4 days. The cells were cultured without abemaciclib for 62 h under time-lapse imaging. Two-way ANOVA, Tukey’s. (H) Cell viability treated with 50 nM abemaciclib or DMSO +/− dox. Data are means ± SEM of four replicates. Two-way ANOVA, Tukey’s. (I) Flow cytometry analysis showing the percentage of SA-β-Gal positive cells. Data are means ± SD of three replicates. Two-way ANOVA, Sidak’s. (J) Colony formation assay. Cells were treated with 50 nM abemaciclib ± dox for 11 days and reseeded without drug. (K) Cell viability of p130KO in dox inducible HA-p21 cells. The cells were treated with 50 nM abemaciclib or DMSO +/− dox. Data are means ± SEM of six replicates. Two-way ANOVA, Tukey’s. See also and .

    Journal: Cancer cell

    Article Title: Long-term breast cancer response to CDK4/6 inhibition defined by TP53-mediated geroconversion

    doi: 10.1016/j.ccell.2024.09.009

    Figure Lengend Snippet: (A) GESA plot with normalized enrichment score (NES) of REACTOME gene sets. NES was analyzed between MCF7 and p53KO treated with 50 nM abemaciclib at day21. REACTOME-E2F-mediated-regulation-of-DNA-replication (top), REACTOME-Transcription-of-E2F-targets-under-negative-control-by-DREAM-complex (middle) and REACTOME-Transcription-of-E2F-targets-under-negative-control-byp107 RBL1-and-p130 RBL2-in-complex-with-HDAC1 (bottom). (B) Immunoblotting of DREAM complex components with 50 nM abemaciclib. (C) Cells were treated with 100 nM abemaciclib for 48 h. Lysates were immunoprecipitated with p130 and IgG antibodies. (D) Cells were treated with 7 days of abamacilib and tested by ChIP followed by real-time PCR. Bar showed technical duplicate. Unpaired, Student’s t test. (E) Western blot results of MCF7 cells and p53KO transduced with doxycycline (dox)-inducible HA-tagged p21. The cells were treated with 50 nM abemaciclib or DMSO +/− dox for 48 h. (F) Dox-inducible HA-p21 MCF7 and p53KO cells were treated with 100 nM abemaciclib and +/− dox for 72 h. Lysates were immunoprecipitated with E2F4 and IgG antibodies. (G) Time course change of the proportion of G1 phase after drug withdrawal. FUCCI labeled MCF7 were treated with 50 nM abemaciclib and FUCCI labeled p53KO cells were treated with or without dox and with 50 nM abemaciclib for 4 days. The cells were cultured without abemaciclib for 62 h under time-lapse imaging. Two-way ANOVA, Tukey’s. (H) Cell viability treated with 50 nM abemaciclib or DMSO +/− dox. Data are means ± SEM of four replicates. Two-way ANOVA, Tukey’s. (I) Flow cytometry analysis showing the percentage of SA-β-Gal positive cells. Data are means ± SD of three replicates. Two-way ANOVA, Sidak’s. (J) Colony formation assay. Cells were treated with 50 nM abemaciclib ± dox for 11 days and reseeded without drug. (K) Cell viability of p130KO in dox inducible HA-p21 cells. The cells were treated with 50 nM abemaciclib or DMSO +/− dox. Data are means ± SEM of six replicates. Two-way ANOVA, Tukey’s. See also and .

    Article Snippet: E2F4 (IP) , Santa Cruz Biotechnology , sc-398543.

    Techniques: Negative Control, Western Blot, Immunoprecipitation, Real-time Polymerase Chain Reaction, Transduction, Labeling, Cell Culture, Imaging, Flow Cytometry, Colony Assay